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How to Reconstitute Your Peptides with BAC Water

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by

Daniel
in

Reconstituting peptides, whether it’s Semaglutide, Tirzepatide, or BPC-157, is a critical process in the world of biochemistry and biotechnology, enabling researchers and clinicians to unlock the full potential of these powerful molecules. Whether you’re working with therapeutic peptides for drug development or conducting experiments in a lab, understanding the nuances of reconstitution can significantly impact your results. Learning how to reconstitute your peptides with BAC water may seem overwhelming at first but you’ll it’s really very straight forward. In this blog post, we’ll explore the essential techniques and best practices for reconstituting peptides effectively using BAC water, ensuring their stability and bioactivity.

Materials Needed

  1. Peptide: Ensure you have the correct peptide whether it’s Tirzepatide or Semaglutide
  2. BAC Water: Bacteriostatic water or BAC water. (All peptide purchases with Biomaxx.shop come with 2ml of BAC water!)
  3. Syringe and Needle: For accurate measurement and transfer of BAC water.
  4. Alcohol Wipes: For sanitizing the vials 
  5. Sterile Vials: For reconstitution and storage.

Steps for Reconstitution

  1. Calculate the Required Volume of BAC Water:
    • Determine the desired concentration of the tripeptide.
    • Use the formula:
      • Volume (mL)=Desired concentration (mg/mL) divided by                            Amount of peptide (mg)
    • Biomaxx Research always gives a 2ml vial of BAC Water 
  2. Prepare the BAC Water:
    • Pop off the cap for the BAC water and peptide vials
    • Use the alcohol wipe to wipe the exposed top of the vials
  3. Reconstitution:
    • Using a syringe, carefully measure the calculated volume of BAC water.
      • Biomaxx.shop gives 2ml vials of BAC water making calculations easier
    • Add the BAC water slowly to the vial containing the peptide, aiming to pour it along the vial walls to minimize foaming.
  4. Mixing:
    • Gently swirl or invert the vial to mix. Avoid vigorous shaking to prevent degradation of the peptide.
    • Allow the solution to sit for a few minutes to ensure complete dissolution.
  5. Check for Complete Dissolution:
    • Inspect the solution for any undissolved particles. If necessary, you can warm the vial slightly (e.g., in a water bath at 37°C) or increase the volume of BAC water.
  6. Storage:
    • If you won’t use the entire volume immediately, aliquot the solution into sterile vials to minimize freeze-thaw cycles.
    • Store the reconstituted peptide at -4°F to 40°F (your fridge should be sufficient), depending on its stability.
  7. Labeling:
    • Clearly label each vial with:
      • Peptide name
      • Concentration
      • Date of reconstitution
    • Luckily the labels from Biomaxx.shop contain the peptide name and concentration already on it!

Important Considerations

  • Stability: Tripeptides can be sensitive to pH and temperature, so handle with care.
  • Sterility: Use aseptic techniques to avoid contamination.
  • Shelf Life: Check the stability information for the specific tripeptide to determine how long it can be stored once reconstituted.

Conclusion

Following this guide will help you effectively reconstitute your peptide using BAC water, ensuring its integrity for subsequent use in experiments or applications. Luckily when you shop through Biomaxx.shop you get a 2ml vial of BAC water with every vial of peptide you purchase!